Pharmaster believes in providing quality lab services that aid in the planning and implementation of efficient and effective research projects. We provide expert services ranging from study-design creation to processing and managing biological samples as well as research data management and bioinformatics services.
Study-design
A detailed and sophisticated study design is the backbone of a research project. We assist our clients in choosing the appropriate clinical study-design and calculating proper sample sizes. After clarifying the objectives and identifying potential errors in the beginning stages of a study, we provide assistance in outlining a research strategy and workflow (overall workflow management, clinical site-specific, wet and dry lab site-specific, etc.) We then prepare a specialized domain model – with a detailed flow chart of sites, activities, entities, their attributes and relationships.
Sample collection
Pharmaster provides pharma and biotech companies with samples collection and analysis services worldwide. We provide assistance in the following steps of research sample collection:
- Obtaining ethical approval
- Collection of human biological samples (human blood and blood fractions, tissue, stool, urine, saliva and buccal cells, skin tissue scrapings).
- Collection of detailed clinical data related to biospecimen procurement.
- Access and collaboration with an elaborate clinical network including large hospitals, medical doctors, nurses, etc.
Biological sample processing:
Our biological sample processing services include:
- DNA / RNA isolation
- Determination of DNA / RNA quality (agarose gel electrophoresis)
- Determination of DNA / RNA quantity (fluorescence-based quantification)
- DNA / RNA synthesis using different PCR techniques (conventional, reverse, nested)
- Methylation-dependent DNA extraction and techniques
- Reverse transcription and cDNA synthesis (one-step and two-step PCR)
Oligonucleotide design
Pharmaster specializes in Oligonucleotide design by providing:
- Gene-specific, short oligonucleotides design on PCR amplification
- Plasmid design
Biobanking
We are also able to provide the following biobanking services:
- Long-term custom storage of biological tissue, DNA and RNA samples and their associated data, organised in a structured and easily analysable format.
- Prospective biospecimen collections for research projects.
High-throughput laboratory experiments:
Pharmaster is able to outsource NGS, microarray and mass spectrometry services to its international partners.
Our sequencing offerings include:
- Targeted or whole genome sequencing for comparative studies
- discovering point mutations
- structural variations
- genotyping to identify candidates for association studies
- Transcriptome analysis using RNA-seq, cDNA based sequencing strategy
- expression level quantification, comparative analysis of different samples
- expression profiles
- transcriptome assembly
- detection of isoform switching
- Small RNA discovery
- discovery and profiling of non-coding RNA
- DNA methylation analysis for epigenetic studies